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Product Info Summary
| SKU: | M03178-5 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Chicken, Equine, Human, Mouse, Pig, Rat |
| Host: | Chicken |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Fibrillarin FBL Antibody
View all Fibrillarin Antibodies
SKU/Catalog Number
M03178-5
Size
100ul
Description
Boster Bio Anti-Fibrillarin FBL Antibody catalog # M03178-5. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Stable at 4℃ for one year
Cite This Product
Anti-Fibrillarin FBL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03178-5)
Host
Chicken
Contents
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full length human fibrillarin expressed in and purified from E. coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03178-5 is reactive to FBL in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Applications
M03178-5 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
33.784kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2,000-1:5,000. IF/ICC: 1:2,000-1:5,000.
Validation Images & Assay Conditions
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Western blot analysis of different cell lysates using chicken pAb to fibrillarin M03178-5, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.
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Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, M03178-5, dilution 1:10,000 in green and costained with mouse mAb to vimentin, 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.
Protein Target Info & Infographic
Gene/Protein Information For FBL (Source: Uniprot.org, NCBI)
Gene Name
FBL
Full Name
rRNA 2'-O-methyltransferase fibrillarin
Weight
33.784kDa
Superfamily
methyltransferase superfamily
Alternative Names
EC 2.1.1,34-kD nucleolar scleroderma antigen; EC 2.1.1.-; EC 2.1.1.37; FIB; FIB1,34 kDa nucleolar scleroderma antigen; fibrillarin; FLRNrRNA 2'-O-methyltransferase fibrillarin; RNA, U3 small nucleolar interacting protein 1; RNU3IP1 FBL FIB, FLRN, Nop1, RNU3IP1 fibrillarin rRNA 2-O-methyltransferase fibrillarin|34 kDa nucleolar scleroderma antigen|34-kD nucleolar scleroderma antigen|RNA, U3 small nucleolar interacting protein 1|U6 snRNA 2-O-methyltransferase fibrillarin|histone-glutamine methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FBL, check out the FBL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibrillarin FBL Antibody (M03178-5)
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No publications found for M03178-5
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Fibrillarin FBL Antibody
Question
Please see the WB image, lot number and protocol we used for placenta using anti-Fibrillarin antibody M03178-5. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-02
Question
I am looking for to test anti-Fibrillarin antibody M03178-5 on rat placenta for research purposes, then I may be interested in using anti-Fibrillarin antibody M03178-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-Fibrillarin antibody M03178-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
We are currently using anti-Fibrillarin antibody M03178-5 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
The anti-Fibrillarin antibody (M03178-5) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-24
